BIO-CHEMISTRY PROJECT TOPICS AND MATERIALS

ANGIOTENSIN CONVERTING ENZYME INHIBITORY ACTIVITY AND ANTIOXIDANT ACTIVITIES OF AQUEOUS EXTRACT OF COMBRETUM MICRANTHUM LEAVES

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ANGIOTENSIN CONVERTING ENZYME INHIBITORY ACTIVITY AND ANTIOXIDANT ACTIVITIES OF AQUEOUS EXTRACT OF COMBRETUM MICRANTHUM LEAVES CHAPTER ONE 1.0 INTRODUCTION 1.1 Background of the Study   The word hypertension is defined as a persistence increase in systemic arterial blood pressure (Sembulingam and Sembuligam, 2006). Clinically, when the systolic pressure remains elevated above 140mmHg and diastolic pressure … Read more

THERMODYNAMICS AND KINETICS OF THERMO-INACTIVATION AND REGENERATION OF PARTIALLY PURIFIED PEROXIDASE FROM Gongronema latifolium LEAVES

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ABSTRACT Peroxidase activity from G. latifolium was done to see whether it could be used in industries. Crude peroxidase was extracted from G. latifolium with 0.05M sodium phosphate buffer of pH 6.0; 70% ammonium sulphate saturation to precipitated protein with the highest G. latifolium peroxidase activity. After gel filtration, two major peaks were seen and … Read more

THE EFFECT OF HYDROGEN PEROXIDE ON PEROXIDASE (EC 1.11.1.7) FROM GONGRONEMA LATIFOLIUM

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ABSTRACT Peroxidase (EC 1.11.1.7) extracted from Gongronema latifolium was purified, on a two-step purification process of ammonium sulphate precipitation followed by dialysis. The enzyme was purified 6.8 fold with a specific activity of 2.04 when o-dianisidine was used as substrate. When the enzyme was subjected to different concentrations of hydrogen peroxide and o-dianisidine, the peak … Read more

THE EFFECT OF ETHANOL EXTRACT OF Theobroma cacao POLYPHENOL ON ISOPROTERENOL-INDUCED MYOCARDIAL INFARCTION IN WISTAR RATS

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ABSTRACT This study investigated the effect of cocoa polyphenol on isoproterenol-induced myocardial infarction in Wistar rats. Polyphenol was extracted from cocoa using a hydroalcohol solvent. Forty albino rats divided into six groups of four rats each were used for the study. Groups 3, 4, 5 and 6 were pretreated with the extract (300, 500 and … Read more

SURVEY OF SODIUM, POTASSIUM, IRON AND CALCIUM CONTENTS OF RETAIL SAMPLES OF SOME PROCESSED FOODS (BREAKFAST CEREALS AND SNACKS) SOLD IN NSUKKA, ENUGU STATE

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TABLE OF CONTENTS Title Page – – – – – – – – I Certification Page – – – – – – – – – ii Dedication – – – – – – – – – iii Acknowledgement – ¬- – – – – – – – iv Abstract – – – – – – … Read more

STUDIES ON THE PHYTOCHEMICAL AND NUTRITIONAL COMPOSITION OF AQUEOUS LEAF EXTRACT OF JUSTICIA CARNEA AND ITS EFFECT ON SOME BIOCHEMICAL PARAMETERS IN ANAEMIC RATS

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ABSTRACT Anaemia is a common blood disorder that affects people of all ages, though people at greater risk are the elderly, young women and infants. Different drugs are used but many depend mainly on plants and herbal products for its treatment. In this study, the aqueous leaf extract of Justicia carnea was used. The percentage … Read more

STUDIES ON THE EFFECT OF AQUEOUS EXTRACT OF MILLETTIA ABOENSIS LEAVES ON LOMOTIL – INDUCED CONSTIPATION IN WISTAR ALBINO RATS

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ABSTRACT Millettia aboensis leaf is a rich source of reducing sugar, tannins, glycosides and flavonoids and has been reported to have medicinal property as well as some physiological potentials. The leaf also has been used by traditional herbalists for general healing of diseases including ulcer and laxatives. This study evaluated the effect of aqueous extract … Read more

RENAL BIOCHEMICAL CHANGES OF PREGNANCY-INDUCED HYPERTENSION IN PREGNANT WOMEN ATTENDING TERTIARY HOSPITALS IN ENUGU STATE

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ABSTRACT Pregnancy induced hypertensive disorders have become very common medical complication in Nigeria with its attendant morbidity and mortality. The morbidity and mortality may be associated with its possible effect on the kidneys which was studied. Results of the electrolyte changes from this study showed that there was non-significant difference (p>0.05) in sodium ion concentration … Read more

PRODUCTION, PARTIAL PURIFICATION AND CHARACTERIZATION OF CELLULASE BY ASPERGILLUS NIGER FROM SUBMERGED FERMENTATION OF GRAPE BAGASSE

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ABSTRACT The increase in agricultural practices has necessitated the judicious use of agricultural wastes into value added products. In this study the ability of selected cellulosic substrate to induce cellulase production by Aspergillus niger and the ability of the induced enzyme to hydrolyze the cellulosic substrate were assessed. The enzyme was produced by submerged fermentation … Read more

PRODUCTION OF Aspergillus niger GLUCOAMYLASE USING GUINEA CORN STARCH AMYLOPECTIN AS THE ONLY CARBON SOURCE

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ABSTRACT This study was aimed at the production of glucoamylase which can be utilised for starch hydrolysis. A fourteen days experimental study was carried out to determine the day of highest glucoamylase activity. Day five and day twelve of the fourteen days experimental study had the highest glucoamylase activity. The specific activity for the crude … Read more